In Vitro Anti Oxidant Activity of Ethanol Extract of Myxopyrum Serratulum A.w.hill
Corresponding Author(s) : N.Elavarasan
kamesh.pharm@gmail.com
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 10 No. 2 (2022): 2022 Volume - 10 Issue - 2
Abstract
Objectives:Free radical induced oxidative stress is involved in the pathogenesis of various diseases and disorders. Antioxidants play an important role against this oxidative stress to protect our body. The present study was carried out to evaluate the in vitro antioxidant properties ofethanolic extract of myxopyrum serratulum (EEMS).
Methods:EEMS was assayed ondifferent in vitro free radical models like DPPH, suproxide radical scavenging assay, hydroxyl scavenging assay.
Result:The extract showed good antioxidant activity in in-vitro free radical scavenging models. Various concentrations ranging from 200 µg/ml and 400 µg/ml of ethanol extract of the leaves of M. serratulum were tested for their antioxidant activity in various in-vitro models. Free radicals were scavenged by the test compounds in a concentration dependent manner within the given range of concentration (IC50) in the DPPH (36.30 ± 1.47%), superoxide scavenging activity (55.30 ± 1.29%), and hydroxide scavenging activity (43.70 ± 2.43%).
Conclusion:All the results of the in vitro antioxidant assays revealed potent antioxidant and free radical scavenging activity of the ethanol extract of myxopyrum serratulum a.w.hill, equivalent to that of standard quercetin and this antioxidant property may be attributed to its high phenolic and flavonoid contents.
Methods:EEMS was assayed ondifferent in vitro free radical models like DPPH, suproxide radical scavenging assay, hydroxyl scavenging assay.
Result:The extract showed good antioxidant activity in in-vitro free radical scavenging models. Various concentrations ranging from 200 µg/ml and 400 µg/ml of ethanol extract of the leaves of M. serratulum were tested for their antioxidant activity in various in-vitro models. Free radicals were scavenged by the test compounds in a concentration dependent manner within the given range of concentration (IC50) in the DPPH (36.30 ± 1.47%), superoxide scavenging activity (55.30 ± 1.29%), and hydroxide scavenging activity (43.70 ± 2.43%).
Conclusion:All the results of the in vitro antioxidant assays revealed potent antioxidant and free radical scavenging activity of the ethanol extract of myxopyrum serratulum a.w.hill, equivalent to that of standard quercetin and this antioxidant property may be attributed to its high phenolic and flavonoid contents.
Keywords
Antioxidant
myxopyrum serratulum
superoxide scavenging activity
ascorbic acid
1.
N.Elavarasan, S.Kameshwaran, J.Amutha Iswarya Devi, V.Srividhya, N.Sriram, R.D.Jaikumar. In Vitro Anti Oxidant Activity of Ethanol Extract of Myxopyrum Serratulum A.w.hill. Int. J. of Allied Med. Sci. and Clin. Res. [Internet]. 2022 Apr. 28 [cited 2026 May 2];10(2):160-4. Available from: https://ijamscr.com/ijamscr/article/view/1173
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References
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1. Yu BP. Cellular defenses against damage from reactive oxidant species.Physiol Rev. 1994;74(1):139-62. doi: 10.1152/physrev.1994.74.1.139, PMID 8295932.
2. Halliwell B, Gutteridge JMC. In Free radicals in biology and medicine.2nd ed. Oxford: Clarendon Press;1988.
3. Lata H, Ahuja GK. Role of free radicals in health and disease. Ind J Physiol Allied Sci.2003;57:124.
4. Contran RS, Kumar V, Collins T.Robbin’s phological basis of diseases.6th ed. Vol. ILtd. Thomson Press.Noida, India;1999.
5. Yu BP, Suescun EA, Yang SY. Effect of age-related lipid peroxidation on membrane fluidity and phospholipase A2: modulation by dietary restriction. Mech Ageing Dev.1992;65(1):17-33. doi: 10.1016/0047-6374(92)90123-u, PMID 1405788.
6. Campbell IC, Abdulla EM. Strategic approaches to in vitro neurotoxicology, in Approaches and methods.Neurotoxicology.1995;495.
7. Marx JL. Oxygen free radicals linked too many diseases.Science.1987;235(4788):529-31. doi: 10.1126/science.3810154, PMID 3810154.
8. Balaji R, Prakash G, deviS. P, AravinthanKM. Antioxidant activity of methanol extract of Ocimum tenuiflorum. Int.Journal of Pharma. Res Dev. 2011;3:20-7.
9. SahaP, Mazumder UK, Haldar PK. In vitroantioxidantactivity of Cucurbita maximaaerial.Parts. 2011;1:1.
10. Aksoy L, Kolay E, A??lönü Y, Aslan Z, Karg?o?lu M. Free radical scavenging activity,total phenolic content, total antioxidant status, of endemic Thermopsis turcica. Saudi J BiolSci. 2013;20(3):235-9. doi: 10.1016/j.sjbs.2013.02.003, PMID 23961240.
11. Sahoo S, Ghosh G, Das D, Nayak S. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigraB.L.Burtt.Asian Pac J Trop Biomed.2013;3(11):871-6. doi: 10.1016/S2221-1691(13)60171-9.
12. RajbharK, DawdaH, MukundanU. Polyphenols: methods of extraction. SciRev ChemCommun. 2015;5(1):1-6.
13. Srividhya V, Sengottuvel T,KanupriyaG, SivakumarA. Antioxidant potential and Phytochemical analysis of fruit extract of Cucurbita pepo.IntJCurrResChemPharmSci.2019;6(3):22-32.
References
1. Yu BP. Cellular defenses against damage from reactive oxidant species.Physiol Rev. 1994;74(1):139-62. doi: 10.1152/physrev.1994.74.1.139, PMID 8295932.
2. Halliwell B, Gutteridge JMC. In Free radicals in biology and medicine.2nd ed. Oxford: Clarendon Press;1988.
3. Lata H, Ahuja GK. Role of free radicals in health and disease. Ind J Physiol Allied Sci.2003;57:124.
4. Contran RS, Kumar V, Collins T.Robbin’s phological basis of diseases.6th ed. Vol. ILtd. Thomson Press.Noida, India;1999.
5. Yu BP, Suescun EA, Yang SY. Effect of age-related lipid peroxidation on membrane fluidity and phospholipase A2: modulation by dietary restriction. Mech Ageing Dev.1992;65(1):17-33. doi: 10.1016/0047-6374(92)90123-u, PMID 1405788.
6. Campbell IC, Abdulla EM. Strategic approaches to in vitro neurotoxicology, in Approaches and methods.Neurotoxicology.1995;495.
7. Marx JL. Oxygen free radicals linked too many diseases.Science.1987;235(4788):529-31. doi: 10.1126/science.3810154, PMID 3810154.
8. Balaji R, Prakash G, deviS. P, AravinthanKM. Antioxidant activity of methanol extract of Ocimum tenuiflorum. Int.Journal of Pharma. Res Dev. 2011;3:20-7.
9. SahaP, Mazumder UK, Haldar PK. In vitroantioxidantactivity of Cucurbita maximaaerial.Parts. 2011;1:1.
10. Aksoy L, Kolay E, A??lönü Y, Aslan Z, Karg?o?lu M. Free radical scavenging activity,total phenolic content, total antioxidant status, of endemic Thermopsis turcica. Saudi J BiolSci. 2013;20(3):235-9. doi: 10.1016/j.sjbs.2013.02.003, PMID 23961240.
11. Sahoo S, Ghosh G, Das D, Nayak S. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigraB.L.Burtt.Asian Pac J Trop Biomed.2013;3(11):871-6. doi: 10.1016/S2221-1691(13)60171-9.
12. RajbharK, DawdaH, MukundanU. Polyphenols: methods of extraction. SciRev ChemCommun. 2015;5(1):1-6.
13. Srividhya V, Sengottuvel T,KanupriyaG, SivakumarA. Antioxidant potential and Phytochemical analysis of fruit extract of Cucurbita pepo.IntJCurrResChemPharmSci.2019;6(3):22-32.
2. Halliwell B, Gutteridge JMC. In Free radicals in biology and medicine.2nd ed. Oxford: Clarendon Press;1988.
3. Lata H, Ahuja GK. Role of free radicals in health and disease. Ind J Physiol Allied Sci.2003;57:124.
4. Contran RS, Kumar V, Collins T.Robbin’s phological basis of diseases.6th ed. Vol. ILtd. Thomson Press.Noida, India;1999.
5. Yu BP, Suescun EA, Yang SY. Effect of age-related lipid peroxidation on membrane fluidity and phospholipase A2: modulation by dietary restriction. Mech Ageing Dev.1992;65(1):17-33. doi: 10.1016/0047-6374(92)90123-u, PMID 1405788.
6. Campbell IC, Abdulla EM. Strategic approaches to in vitro neurotoxicology, in Approaches and methods.Neurotoxicology.1995;495.
7. Marx JL. Oxygen free radicals linked too many diseases.Science.1987;235(4788):529-31. doi: 10.1126/science.3810154, PMID 3810154.
8. Balaji R, Prakash G, deviS. P, AravinthanKM. Antioxidant activity of methanol extract of Ocimum tenuiflorum. Int.Journal of Pharma. Res Dev. 2011;3:20-7.
9. SahaP, Mazumder UK, Haldar PK. In vitroantioxidantactivity of Cucurbita maximaaerial.Parts. 2011;1:1.
10. Aksoy L, Kolay E, A??lönü Y, Aslan Z, Karg?o?lu M. Free radical scavenging activity,total phenolic content, total antioxidant status, of endemic Thermopsis turcica. Saudi J BiolSci. 2013;20(3):235-9. doi: 10.1016/j.sjbs.2013.02.003, PMID 23961240.
11. Sahoo S, Ghosh G, Das D, Nayak S. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigraB.L.Burtt.Asian Pac J Trop Biomed.2013;3(11):871-6. doi: 10.1016/S2221-1691(13)60171-9.
12. RajbharK, DawdaH, MukundanU. Polyphenols: methods of extraction. SciRev ChemCommun. 2015;5(1):1-6.
13. Srividhya V, Sengottuvel T,KanupriyaG, SivakumarA. Antioxidant potential and Phytochemical analysis of fruit extract of Cucurbita pepo.IntJCurrResChemPharmSci.2019;6(3):22-32.