Comparative Study of Anti Bacterial Activity of Barks, Leaves and Flesh Extracts of Moringa Oleifera L
Corresponding Author(s) : S. Sundar
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 2 No. 3 (2014): 2014 Volume 2- Issue -3
Abstract
In the present study, comparative anti bacterial activity of barks, leaves and flesh extracts of Moringa oleifera L was evaluated with different pathogenic gram positive and gram negative microorganisms. Twelve extracts of barks, leaves and flesh of Moringa oleifera L were prepared with four solvent such as alcohol, ethyl acetate, benzene and acetone. The combinations of extracts were prepared in 1:1 ratio. Antimicrobial activity was evaluated by agar disc diffusion method. All the extracts showed the anti microbial activity against the microorganisms. According to the zone of inhibition observed in the entire agar plates, the ethyl acetate extracts of Moringa oleifera (Leaf and Bark), (Bark and Flesh) showed maximum zone of inhibitions 20 mm, 17 mm against Micrococcus luteus respectively. The alcoholic extract (Leaf and Flesh) showed zone of inhibition 19 mm against Bacillus Subtilis and (Bark and Flesh) extract showed zone of inhibition 18 mm against the pseudomonas aeuroginosa. The acetone extract of Moringa oleifera showed lowest antimicrobial activity when compare to the other solvents extracts. From these studies concluded that, ethyl acetate extract of Moringa oleifera showed good antimicrobial activity than other extracts. Further the analysis of active ingredients present in this plant responsible for antimicrobial activity is important for the further development of new drugs.
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[1] Satyavati GV and Gupta AK, 1987. Medicinal plants of India. ICMR, New Delhi, Vol.2, 272-278.
[2] Nunn, John 2002 Ancient Egyptian medicine. University of oklahama press.p.151.ISBN 97-0-8061-350-5.
[3] Thomson W. A. R. 1978. The kingdom of plants in Medicines from the Earth. Ed (McGraw-Hill Book Co. New York, N.Y), p 208.
[4] Ackerknect, Erwin 1982.A Short History of Medicine. JHU press. P.151ISBN 978-0-8018-2726-6.
[5] Serafini M., 1993. Synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape 2S albumins and by barley trypsin inhibitors. Plant Physiol 103:1311–1319
[6] Schultz J. C. 1998. Effect of selected monoterpenes on methane oxidation, denitrification, and aerobic metabolism by bacteria in pure culture. Appl. Environ Microbiol. 64:520– 525.
[7] Delaveau, P. & Boiteau P. 1980. Huiles à interet pharmacologique, cosmetologique et dietique. IV. Huiles de Moringa oleifera Lamk. et de M. Drouhardii Jumelle. Plantes medicinales et phytotherapie. 14, 29-33.
[8] Dubey NK, Kumar R, Tipathi P.Global promotion of herbal medicine: India’s opportunity. Currrent. Science. (India) 86:37-41.
[9] Sowmiya S, Soundara Pandian P, Rajan S 2009. Bioactive studies of Moringa oleifera against bacteria isolated from urine samples. Curr. Res. J. Biol.sci.1:139-143.
[10] Manolova N. 1991. ?-(1, 3) - and ?-(1, 6)-d-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections in vitro. Antimicrob. Agents Chemother. 35:410–416.
[11] Eilert U, Wolters B, Nahrsted A, 1981. The antibiotic Principle of seeds of Moringa oleifera and Moringa stenopetla. Plant Med. 42:55-61.
[12] Kaimal.p,Bhatt.r 1989. Antibacterial and bactericidal activities of Japanese green tea. Jpn. J Bacteriol. 45:561–566.
[13] Bauer, A.W, W.M.M. Kirby, J.C. Sherris and M. Turck: Antibiot ic susceptibility testing by a standardized single disc method. Am. J.Clin. Pathol 1966, 45, 493-496.
[14] Akinyanju J.A Adetitum D.O, Araoye, Anibijuwon I. 2013. The antimicrobial effects of the leaf extracts of Moringa oleifera on selected clinical bacteria isolated.Agro research.1:95-113.
References
[2] Nunn, John 2002 Ancient Egyptian medicine. University of oklahama press.p.151.ISBN 97-0-8061-350-5.
[3] Thomson W. A. R. 1978. The kingdom of plants in Medicines from the Earth. Ed (McGraw-Hill Book Co. New York, N.Y), p 208.
[4] Ackerknect, Erwin 1982.A Short History of Medicine. JHU press. P.151ISBN 978-0-8018-2726-6.
[5] Serafini M., 1993. Synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape 2S albumins and by barley trypsin inhibitors. Plant Physiol 103:1311–1319
[6] Schultz J. C. 1998. Effect of selected monoterpenes on methane oxidation, denitrification, and aerobic metabolism by bacteria in pure culture. Appl. Environ Microbiol. 64:520– 525.
[7] Delaveau, P. & Boiteau P. 1980. Huiles à interet pharmacologique, cosmetologique et dietique. IV. Huiles de Moringa oleifera Lamk. et de M. Drouhardii Jumelle. Plantes medicinales et phytotherapie. 14, 29-33.
[8] Dubey NK, Kumar R, Tipathi P.Global promotion of herbal medicine: India’s opportunity. Currrent. Science. (India) 86:37-41.
[9] Sowmiya S, Soundara Pandian P, Rajan S 2009. Bioactive studies of Moringa oleifera against bacteria isolated from urine samples. Curr. Res. J. Biol.sci.1:139-143.
[10] Manolova N. 1991. ?-(1, 3) - and ?-(1, 6)-d-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections in vitro. Antimicrob. Agents Chemother. 35:410–416.
[11] Eilert U, Wolters B, Nahrsted A, 1981. The antibiotic Principle of seeds of Moringa oleifera and Moringa stenopetla. Plant Med. 42:55-61.
[12] Kaimal.p,Bhatt.r 1989. Antibacterial and bactericidal activities of Japanese green tea. Jpn. J Bacteriol. 45:561–566.
[13] Bauer, A.W, W.M.M. Kirby, J.C. Sherris and M. Turck: Antibiot ic susceptibility testing by a standardized single disc method. Am. J.Clin. Pathol 1966, 45, 493-496.
[14] Akinyanju J.A Adetitum D.O, Araoye, Anibijuwon I. 2013. The antimicrobial effects of the leaf extracts of Moringa oleifera on selected clinical bacteria isolated.Agro research.1:95-113.