Anti-Inflammatory Activity of Pupalia Lappacea L. Juss
Corresponding Author(s) : A.Tamil Selvan
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 2 No. 2 (2014): 2014 Volume 2- Issue -2
Abstract
Pupalia lappacea (L) Juss is an erect shrub used in folklore medicine to treat bone fractures and in inflammatory conditions. Methanolic extract of aerial parts shown is claimed in traditional medicine that the leaves of the plant are used in the treatment of inflammation. In the present study, the methanolic extract of Pupalia lappacea was screened for its anti-inflammatory activity using carageenan induced rat paw edema egg white induced paw oedema models. The methanolic extract at the dose of 200 mg/kg p.o exhibited significant anti-inflammatory activity in carrageenan induced paw edema model (p<0.01). In egg white induced model, methanolic extract at the dose of 200 mg/kg inhibited paw oedema significantly (p<0.01) indicating that both test samples inhibit the increase in number of fibroblasts and synthesis of collagen and mucopolysaccharides during prostaglandin formation during the inflammation. These experimental results have established a pharmacological evidence for the folklore claim of the drug to be used as an anti inflammatory agent. HPTLC analysis of the extract shows the presence of gallic acid 1.24mg/ml, ferulic acid 2.00mg/ml, chlorogenic acid 46.25mg/ml and rutin 7.02mg/ml of the extract which were responsible for the claimed anti-inflammatory action in the animal models studied.
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References
[2] Reddy, S.C., Reddy, K.N., Murthy, E. N. and Raju, U. S. Traditional Medicinal plants in Seshachalam Hills, Andhra Pradesh, India, Journal of Medicinal Plants Research, 3, 2009, 408-412.
[3] Kokate, C. K., Practical Pharmacognosy, Vallabh Prakashan, New Delhi, India, fourth ed., 1994, 107-109.
[4] Harborne, J. B., Phytochemical Methods, A guide to modern techniques of plant analysis, Springer Pvt Ltd, New Delhi, India, third ed., 2007, 45.
[5] Armatu et al, Evaluation of antioxidant and free radical scavenging potential of some Lamiaceae species growing in Romania, Vol.15, No.3, 2010, 5274, 5280.
[6] Ecobichon, D.J, The Basis of Toxicology Testing. CRC Press, New York, 199743–86.
[7] Winter, C.A., Porter, C.A., Effect of alterations in side chain upon anti-in?ammatory and liver glycogen activities of hydrocortisone esters. J. Am. Pharm. Assoc. 46, 1957, 515–519.
[8] Winter, C.A., Risley, E.A., Nuss, G.W., 1962. Carrageenan-induced edema in hind paws of rats as an assay for anti-in?ammatory drugs. Proc. Soc. Exp. Biol. Med. 11, 544–547.
[9] Savitree, M., Isara, P., Nittaya, S.L., Worapan, S. Radical Scavenging Activity and Total Phenolic Content of Medicinal Plants Used in Primary Health Care. Journal of Pharm. Science 9(1), 2004, 32-35.
[10] Zhishen, J, Mengcheng, T., Jianming, W, The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 1999, 555-559.