Study on Anti anxiety activity of ALERT – A Poly herbal formulation in Experimental animal models

Abstract

The present study was performed to evaluate the anti anxiety activity of ALERT – a Poly herbal formulation in experimental animal model. Anti anxiety activity was determined in experimental Swiss Albino Mice. The test was carried out by Elevated plus maze, Light/dark exploration test, Open field tests, Rota-rod test and Hole board test. In the Elevated Plus maze the total number of arm entries and the time spent in open and enclosed arms were recorded. In Light and dark exploration tests, the total number of arm entries and the time spent in light and dark chambers were recorded. In Open field tests the number Ambulation, Rearing, Self grooming, activity in center were recorded. In Hole board test the number and duration of head-dippings were recorded. In Rota-rod tests, the loco motor activity with fall in time was recorded. The animals were treated with three doses of the Poly herbal formulation ALERT, of doses low (50mg/kg), moderate (150mg/kg) and high (200mg/kg). In Elevated Plus maze significant increase in the number of entries and duration of stay in open arm was observed. In Light/ Dark exploration tests, significant increase in the time spent and numbers of entries in light compartment were observed. In Open field tests, there was significant increase in the number of squares crossed, number of crossings and rearing. In hole board tests, has shown a significant reduction in locomotion. In Rota- rod tests, significantly decreased time spent on revolving Rota-rod. The study revealed that the Poly herbal formulation ALERT possess significant anti-anxiety effect.