Lipid profile and hormone Ssensitive lipase [HSL] gene variant in Nigeria women with breast cancer

Abstract

Background

Worldwide, breast cancer is the most common cancer in women, being one of the leading causes of death among women and men. Emerging evidence suggests the role of lipid in the development of breast cancer. It becomes a great concern for the modern man and medical curiosity to further investigate alteration in lipid profile and hormone sensitive lipases genes among breast cancer patients in Nigeria.

Aim and Objective

This study was designed to investigate and compare the atherogenic indices and polymorphism of Hormone Sensitive Lipase gene in individuals with Breast cancer and healthy subjects (non-cancerous subject).

Materials and Methods

A total of 81women were in this study and were divided into two groups. Blood samples were collected from 50 patients with breast cancer and 31 normal controls for lipid profiles (T-CHOL, TG, HDL-C and LDL-C). The atherogenic indices (Lipid profile) were estimated spectrophotometrically and the Hormone Sensitive Lipase alleles were determined using polymerase chain reaction and Restriction Fragment length polymorphism methods.

Results

The results of Total cholesterol, Triglyceride and Low Density Lipoprotein in the breast cancer group were statistically significantly increased as compared with normal controls group (p<0.05) whereas no significant difference was seen statistically in the mean values of HDL,BMI,AGE(p>0.05) when test was compared with control group. In the comparison of the frequency of Hormone Sensitive Lipase gene in both Breast cancer and Non-breast cancer subjects, the result shows a higher frequency of mutant HSL in breast cancer cases compared with controls although the difference between the frequency in cases and control was not statistically significant.