Analytical Method Development and Method Validation of L- Carnosine in Tablet Dosage Form by UV Spectrophotometer

Abstract

L‑Carnosine is a naturally occurring dipeptide widely used in pharmaceutical and nutraceutical formulations due to its antioxidant and anti-aging properties. The present study focuses on the development and validation of a simple, rapid, and economical analytical method for the quantitative estimation of L-Carnosine in tablet dosage form using UV–Visible Spectrophotometry. The method was developed using a suitable solvent system and the absorbance of L-Carnosine was measured at its maximum wavelength. Then the developed method is applied on the quantification of marketed formulation. The developed method was validated according to the guidelines of ICH and the parameters such as linearity, solution stability, accuracy, precision, specificity, limit of detection, limit of quantification, and robustness. The results indicated that the method is reliable and reproducible for the routine analysis of L-Carnosine in tablet formulations by UV Spectrometry. This method is suitable for this drug in pharmaceutical industries.