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Enantiomeric separation of drugs by HPLC
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 13 No. 2 (2025): 2025 Volume -13 - Issue 2
Abstract
The need to separate drug enantiomers isn't just a technical challenge it s a crucial aspect of making medicines safer and more effective. High-Performance Liquid Chromatography (HPLC) has proven itself as a reliable tool for achieving precise and reproducible enantiomeric separations. This review explores various strategies for enantiomeric separation via HPLC, from the use of chiral stationary phases to clever tweaks in the mobile phase. We ll also dive into real-world applications, the hurdles researchers face, and how the field is evolving.
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- Okamoto, Y., & Yashima, E. (1998). Polysaccharide Derivatives for Chromatographic Separation of Enantiomers. Angewandte Chemie International Edition, 37(1-2), 102-108.
- Wainer, I. W. (1993). Protein-based chiral stationary phases for liquid chromatography. Journal of Chromatography A, 666(1-2), 3-26.
- Szakács, Z., & Biczók, L. (2004). Cyclodextrin-based chiral selectors in HPLC. Journal of Chromatography B, 804(1), 1-20.
- Ward, T. J., & Ward, K. D. (2012). Chiral separations: A review of current techniques and future trends. Journal of Chromatography A, 1269, 3-17.
- Lindner, W., & Rimböck, K. (1993). Macrocyclic glycopeptide-based chiral stationary phases. Analytical Chemistry, 65(10), 1258-1270.
- Armstrong, D. W. (1987). Use of liquid chromatographic mobile phase modifiers for enantiomeric separations. Analytical Chemistry, 59(17), 2240-2248.
- Ali, I., & Aboul-Enein, H. Y. (2004). Chiral separations by liquid chromatography and related technologies. CRC Press.
- Gübitz, G., & Schmid, M. G. (2001). Chiral separation principles in high-performance liquid chromatography and capillary electrophoresis. Journal of Chromatography A, 906(1-2), 123-155.
- Ali, I., & Gupta, V. K. (2006). Advances in chiral separations by liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 40(3), 675-690.
- Chankvetadze, B. (2012). Enantioselective separation in high-performance liquid chromatography. Analytical Chemistry, 84(3), 760-770.
- Scriba, G. K. E. (2016). Chiral analysis of drugs: Applications and challenges. Analytical and Bioanalytical Chemistry, 408(3), 701-720.
- Yang, Y., et al. (2017). Strategies for the separation of enantiomers in pharmaceutical analysis. TrAC Trends in Analytical Chemistry, 91, 58-71.
- Gal, J. (2002). Advances in chiral separations by liquid chromatography. Analytical Chemistry, 74(5), 1041-1051.
References
Okamoto, Y., & Yashima, E. (1998). Polysaccharide Derivatives for Chromatographic Separation of Enantiomers. Angewandte Chemie International Edition, 37(1-2), 102-108.
Wainer, I. W. (1993). Protein-based chiral stationary phases for liquid chromatography. Journal of Chromatography A, 666(1-2), 3-26.
Szakács, Z., & Biczók, L. (2004). Cyclodextrin-based chiral selectors in HPLC. Journal of Chromatography B, 804(1), 1-20.
Ward, T. J., & Ward, K. D. (2012). Chiral separations: A review of current techniques and future trends. Journal of Chromatography A, 1269, 3-17.
Lindner, W., & Rimböck, K. (1993). Macrocyclic glycopeptide-based chiral stationary phases. Analytical Chemistry, 65(10), 1258-1270.
Armstrong, D. W. (1987). Use of liquid chromatographic mobile phase modifiers for enantiomeric separations. Analytical Chemistry, 59(17), 2240-2248.
Ali, I., & Aboul-Enein, H. Y. (2004). Chiral separations by liquid chromatography and related technologies. CRC Press.
Gübitz, G., & Schmid, M. G. (2001). Chiral separation principles in high-performance liquid chromatography and capillary electrophoresis. Journal of Chromatography A, 906(1-2), 123-155.
Ali, I., & Gupta, V. K. (2006). Advances in chiral separations by liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 40(3), 675-690.
Chankvetadze, B. (2012). Enantioselective separation in high-performance liquid chromatography. Analytical Chemistry, 84(3), 760-770.
Scriba, G. K. E. (2016). Chiral analysis of drugs: Applications and challenges. Analytical and Bioanalytical Chemistry, 408(3), 701-720.
Yang, Y., et al. (2017). Strategies for the separation of enantiomers in pharmaceutical analysis. TrAC Trends in Analytical Chemistry, 91, 58-71.
Gal, J. (2002). Advances in chiral separations by liquid chromatography. Analytical Chemistry, 74(5), 1041-1051.