Antimicrobial effect of emblica officinalis and cayratiapedata against helicobacter pylori
Corresponding Author(s) : G.Shanthi
drshanthig@yahoo.com
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 2 No. 4 (2014): 2014 Volume 2- Issue -4
Abstract
Helicobacter pylori have been attracting worldwide attention as a major factor or cofactor responsible for gastritis and peptic ulcer. To eradicate this micro-organism, numerous regimes containing various antimicrobial agents have been suggested. However, H.pylori antimicrobial resistance is a leading factor to treatment failure and recurrence of the disease. Traditional medicine based on herbal remedies has always played key role in the health system of many countries. Clinical research has confirmed the efficacy of several medicinal plants for the treatment of gastro-duodenal disease, and basic scientific research has uncovered mechanisms to explain their therapeutic effects, hence we have compared two plant extracts such as Emblica officinalis and Cayratiapedata for the antimicrobial activity of H.pylori. Thirty patients attending the OPD of Rajah Muthiah medical college and hospital, for Acid peptic disease (ulcer and non ulcer type) were subjected to endoscopy at RMMCH, Annamalai University, Chidambaram. Antral biopsies were collected from the patients with gastritis, duodentis, duodenal ulcer, non- ulcer dyspepsia and gastric carcinoma. All the biopsy specimens were processed for H.pylori by Gram´s stain, urease test and culture. All the positive culture was processed for the Antimicrobial resistance by Disk Diffusion Method. The minimum inhibition zone diameter of Emblica officinalis and Cayaratiapedata were detected. Emblica officinalis and Cayratiapedata exhibited potential of in-vitro antimicrobial activity against H.pylori, which suggests that it may be useful for the treatment of H.pylori infection.
Keywords
Emblica officinalis
Cayratiapedata
H.pylori
1.
G.Shanthi. Antimicrobial effect of emblica officinalis and cayratiapedata against helicobacter pylori. Int. J. of Allied Med. Sci. and Clin. Res. [Internet]. 2020 Aug. 2 [cited 2025 Nov. 6];2(4):453-8. Available from: https://ijamscr.com/ijamscr/article/view/118
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References
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[1] A.Leo Stanley et al., V. Alex Ramani , A. Ramachandran. Phytochemical Screening and
[2] GC-MSStudies on the Ethanolic Extract of Cayratiapedata Int.J.Pharm.Phytopharmacol Res. 2011, 1(3): 112-116.
[3] Alzoerky, N.S. and K. Nakahara, 2003. Antibacterial Otegbayo, C. Monica, R. Haas and G. Rieder, 2010. activity of extracts from some edible plants Diagnostic methods for the detection of Helicobacter commonly consumed in Asia. International Journal of pylori in Nigeria. Tropical Gastroenterology, Food Microbiology, 80: 223-230.
[4] Activity of Cayratiapedataleaves on Wister albino rats, Scholars research library. Der PharmaciaLettre, 2011,3 (2) : 433-437.
[5] Covacci, A., Telford, J.L., Giudice, G.D., Parsonnet, J., Rappuoli, R., 1999. Helicobacterpylori virulence and genetic geography. Science 284, 1328–1333.
[6] Crowe SE, Alvarez L, Dytoc M, et al. Expression ofinterleukin-8 and CD54 by human gastric epithelium afterHelicobacter pylori infection in vitro. Gastroenterology 1995;108: 65-74.
[7] Crabtree JE. Gastric mucosal inflammatory responses toHelicobacter pylori. Aliment PharmacolTher 1996; 10: 29- 23.Crowe SE, Alvarez L, Dytoc M, et al. Expression ofinterleukin-8 and CD54 by human gastric epithelium afterHelicobacter pylori infection in vitro. Gastroenterology 1995;108: 65-74.
[8] Gurbuz I, Akyuz C, Yesilada E, Sener B. Anti-ulcerogenic effect of Momordica charantia L. fruits on various ulcer models in rats. J Ethnopharmacol 2000; 71: 77-82.
[9] Hentschel, E., Brandstatter, G., Dragsics, B., Hirschil, A.M., Nemec, H., Schutze, K.,Taufer, M., Wurzer, H., 1993. Effect of ranitidine and amoxicillin plus metronidazoleon the eradication of Helicobacter pylori and the recurrence of duodenalulcer. The New England Journal of Medicine 328, 308–312.
[10] Hentschel, E., Brandstatter, G., Dragsics, B., Hirschil, A.M., Nemec, H., Schutze, K.,Taufer, M., Wurzer, H., 1993. Effect of ranitidine and amoxicillin plus metronidazoleon the eradication of Helicobacter pylori and the recurrence of duodenalulcer. The New England Journal of Medicine 328, 308–312
[11] Jose, J.K., Y. Kuttan and R. Kuttan, 2001. Antitumor activity of Emblica officinalis . Journal of Ethnopharmacology, 75: 65-69.
[12] Lin, Y.T., Y.I. Kwon, R.G. Labbee and K. Shett, 2005. virus infection on plant pigments. Experimental Inhibition of Heliobacter pylori and associated Biology, 29: 1165-1167. urease by oregano and cranberry phytochemical synergies. Applied and Environmental Microbiology, 71: 8558-8564.
[13] Ndip, R.N., Tarkang, A.E.M., Mbullah, S.M., Luma, H.N., Malongue, A., Ndip, L.M.,Nyongbela, K., Wirmum, C., Efange, S.M.N., 2007. In vitro anti-Helicobacter pyloriactivity of extracts of selected medicinal plants from North West Cameroon.Journal of.Ethnopharmacology 114, 452–457
[14] P.Karthik,et al., 1P.Amudha, 2J.SrikanthStudy on phytochemical profile and anti-ulcerogenic effect of Cayratia pedataLamin albino wistar ratsPharmacologyonline 2: 1017-1029 (2010) Karthik et al.
[15] Park S, Lee DK, Yang CH. Inhibition of fos-jun-DNA complex formation by dihydroguaiaretic acid and in vitro cytotoxic effects on cancer cells. Cancer Letters 1998; 27:
[16] Patil, S. G. Honrao, B. K. Journal of Economic and Taxonomic Botany, 2000, 24(3): 688-694
[17] Pounder RE, Ng D.The prevalence of Helicobacter pylori indifferent countries.Aliment PharmacolTher 1995; 9 (Suppl2): 33-9.
[18] Phillipson, J.D. and M.J.O. Neill, 1987. New leads to the treatment of protozoal infections based on natural product molecules. Acta pharmaceutica Nordica, 1:131-144.
[19] Sepulveda, A.R., Coelho, L.G.V., 2002. Helicobacter pylori and gastric malignancies.Helicobacter 7, 37–42.
[20] Shin, I.S., Masuda, H., Naohide, K., 2004. Bactericidal activity of wasabi (Wasbiajaponica) against Helicobacter pylori. Intentional Journal of Food Microbiology94, 255–261.
[21] Sullivan, P.B., Thomas, J.E., Wight, D.G., et al., 1990.H. pylori infection in Gambian childrenwith chronic diarrhea and malnutrition. Archives of Disease in Childhood65, 189–191.Thompson, C.J., Ernst, E., 2002. Systemic review: herbal medicinal productsfor non-ulcer dyspepsia. Alimentary Pharmacology and Therapeutics 16,1689–1699
[22] Scartezzini, P., F. Antognoni, M.A. Raggi, F. Poli and C. Sabbioni, 2006. Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of Emblica officinalis Gaertn. Journal of Ethnopharmacology, 104: 113-118.
[23] Sairam, K., C.V. Rao, B.M. Dora, K.K. Vijay, V.K. Agarwal and R.K. Goel, 2002. Antiulcerogenic effect of methanolic extract of Emblica officinalis : An experimental study. Journal of Ethnopharmacology, 82: 1-9.
[24] VeeradassRajendran, Indumathy S and Gopal V, Anti-nociceptive activity of Cayratiapedatainexperimental animal models, Journal of Pharmacy Research, 2011,4(3):852-853.
[25] VeeradassRajendran, Rathinambal V and Gopal V, A preliminary study on antiinflammatoryactivity of Cayratiapedataleaves on Wister albino rats, Scholars research library. Der PharmaciaLettre, 2011,3 (2) : 433-437.
[26] Voravathikuchai, S.P. and H. Mitchell, 2008. Inhibitory and killing Activities of MedicinalPlants against Multiple Antibiotic-resistant Helicobacter pylori. Journal of Health Science,54: 81-88.
[27] Wang, Y.C., Huang, T.L., 2005. Screening of anti-Helicobacter pylori herbs derivingfrom Taiwanese folk medicinal plants. FEMS Immunology and Medical Microbiology43, 295–300.
[28] WarrenJR,Marshall BJ. Unidentified curve bacilli on gastric epithelium in active gastricLancet 1983; 1; 1273-1275.
[29] Yesilada, E., Gurbuz, I., Shibata, H., 1999. Screening of Turkish anti-ulcerogenic folk remedies for anti- Helicobacter pylori activity.Journal of Ethnopharmacology 66,289–293.
References
[1] A.Leo Stanley et al., V. Alex Ramani , A. Ramachandran. Phytochemical Screening and
[2] GC-MSStudies on the Ethanolic Extract of Cayratiapedata Int.J.Pharm.Phytopharmacol Res. 2011, 1(3): 112-116.
[3] Alzoerky, N.S. and K. Nakahara, 2003. Antibacterial Otegbayo, C. Monica, R. Haas and G. Rieder, 2010. activity of extracts from some edible plants Diagnostic methods for the detection of Helicobacter commonly consumed in Asia. International Journal of pylori in Nigeria. Tropical Gastroenterology, Food Microbiology, 80: 223-230.
[4] Activity of Cayratiapedataleaves on Wister albino rats, Scholars research library. Der PharmaciaLettre, 2011,3 (2) : 433-437.
[5] Covacci, A., Telford, J.L., Giudice, G.D., Parsonnet, J., Rappuoli, R., 1999. Helicobacterpylori virulence and genetic geography. Science 284, 1328–1333.
[6] Crowe SE, Alvarez L, Dytoc M, et al. Expression ofinterleukin-8 and CD54 by human gastric epithelium afterHelicobacter pylori infection in vitro. Gastroenterology 1995;108: 65-74.
[7] Crabtree JE. Gastric mucosal inflammatory responses toHelicobacter pylori. Aliment PharmacolTher 1996; 10: 29- 23.Crowe SE, Alvarez L, Dytoc M, et al. Expression ofinterleukin-8 and CD54 by human gastric epithelium afterHelicobacter pylori infection in vitro. Gastroenterology 1995;108: 65-74.
[8] Gurbuz I, Akyuz C, Yesilada E, Sener B. Anti-ulcerogenic effect of Momordica charantia L. fruits on various ulcer models in rats. J Ethnopharmacol 2000; 71: 77-82.
[9] Hentschel, E., Brandstatter, G., Dragsics, B., Hirschil, A.M., Nemec, H., Schutze, K.,Taufer, M., Wurzer, H., 1993. Effect of ranitidine and amoxicillin plus metronidazoleon the eradication of Helicobacter pylori and the recurrence of duodenalulcer. The New England Journal of Medicine 328, 308–312.
[10] Hentschel, E., Brandstatter, G., Dragsics, B., Hirschil, A.M., Nemec, H., Schutze, K.,Taufer, M., Wurzer, H., 1993. Effect of ranitidine and amoxicillin plus metronidazoleon the eradication of Helicobacter pylori and the recurrence of duodenalulcer. The New England Journal of Medicine 328, 308–312
[11] Jose, J.K., Y. Kuttan and R. Kuttan, 2001. Antitumor activity of Emblica officinalis . Journal of Ethnopharmacology, 75: 65-69.
[12] Lin, Y.T., Y.I. Kwon, R.G. Labbee and K. Shett, 2005. virus infection on plant pigments. Experimental Inhibition of Heliobacter pylori and associated Biology, 29: 1165-1167. urease by oregano and cranberry phytochemical synergies. Applied and Environmental Microbiology, 71: 8558-8564.
[13] Ndip, R.N., Tarkang, A.E.M., Mbullah, S.M., Luma, H.N., Malongue, A., Ndip, L.M.,Nyongbela, K., Wirmum, C., Efange, S.M.N., 2007. In vitro anti-Helicobacter pyloriactivity of extracts of selected medicinal plants from North West Cameroon.Journal of.Ethnopharmacology 114, 452–457
[14] P.Karthik,et al., 1P.Amudha, 2J.SrikanthStudy on phytochemical profile and anti-ulcerogenic effect of Cayratia pedataLamin albino wistar ratsPharmacologyonline 2: 1017-1029 (2010) Karthik et al.
[15] Park S, Lee DK, Yang CH. Inhibition of fos-jun-DNA complex formation by dihydroguaiaretic acid and in vitro cytotoxic effects on cancer cells. Cancer Letters 1998; 27:
[16] Patil, S. G. Honrao, B. K. Journal of Economic and Taxonomic Botany, 2000, 24(3): 688-694
[17] Pounder RE, Ng D.The prevalence of Helicobacter pylori indifferent countries.Aliment PharmacolTher 1995; 9 (Suppl2): 33-9.
[18] Phillipson, J.D. and M.J.O. Neill, 1987. New leads to the treatment of protozoal infections based on natural product molecules. Acta pharmaceutica Nordica, 1:131-144.
[19] Sepulveda, A.R., Coelho, L.G.V., 2002. Helicobacter pylori and gastric malignancies.Helicobacter 7, 37–42.
[20] Shin, I.S., Masuda, H., Naohide, K., 2004. Bactericidal activity of wasabi (Wasbiajaponica) against Helicobacter pylori. Intentional Journal of Food Microbiology94, 255–261.
[21] Sullivan, P.B., Thomas, J.E., Wight, D.G., et al., 1990.H. pylori infection in Gambian childrenwith chronic diarrhea and malnutrition. Archives of Disease in Childhood65, 189–191.Thompson, C.J., Ernst, E., 2002. Systemic review: herbal medicinal productsfor non-ulcer dyspepsia. Alimentary Pharmacology and Therapeutics 16,1689–1699
[22] Scartezzini, P., F. Antognoni, M.A. Raggi, F. Poli and C. Sabbioni, 2006. Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of Emblica officinalis Gaertn. Journal of Ethnopharmacology, 104: 113-118.
[23] Sairam, K., C.V. Rao, B.M. Dora, K.K. Vijay, V.K. Agarwal and R.K. Goel, 2002. Antiulcerogenic effect of methanolic extract of Emblica officinalis : An experimental study. Journal of Ethnopharmacology, 82: 1-9.
[24] VeeradassRajendran, Indumathy S and Gopal V, Anti-nociceptive activity of Cayratiapedatainexperimental animal models, Journal of Pharmacy Research, 2011,4(3):852-853.
[25] VeeradassRajendran, Rathinambal V and Gopal V, A preliminary study on antiinflammatoryactivity of Cayratiapedataleaves on Wister albino rats, Scholars research library. Der PharmaciaLettre, 2011,3 (2) : 433-437.
[26] Voravathikuchai, S.P. and H. Mitchell, 2008. Inhibitory and killing Activities of MedicinalPlants against Multiple Antibiotic-resistant Helicobacter pylori. Journal of Health Science,54: 81-88.
[27] Wang, Y.C., Huang, T.L., 2005. Screening of anti-Helicobacter pylori herbs derivingfrom Taiwanese folk medicinal plants. FEMS Immunology and Medical Microbiology43, 295–300.
[28] WarrenJR,Marshall BJ. Unidentified curve bacilli on gastric epithelium in active gastricLancet 1983; 1; 1273-1275.
[29] Yesilada, E., Gurbuz, I., Shibata, H., 1999. Screening of Turkish anti-ulcerogenic folk remedies for anti- Helicobacter pylori activity.Journal of Ethnopharmacology 66,289–293.
[2] GC-MSStudies on the Ethanolic Extract of Cayratiapedata Int.J.Pharm.Phytopharmacol Res. 2011, 1(3): 112-116.
[3] Alzoerky, N.S. and K. Nakahara, 2003. Antibacterial Otegbayo, C. Monica, R. Haas and G. Rieder, 2010. activity of extracts from some edible plants Diagnostic methods for the detection of Helicobacter commonly consumed in Asia. International Journal of pylori in Nigeria. Tropical Gastroenterology, Food Microbiology, 80: 223-230.
[4] Activity of Cayratiapedataleaves on Wister albino rats, Scholars research library. Der PharmaciaLettre, 2011,3 (2) : 433-437.
[5] Covacci, A., Telford, J.L., Giudice, G.D., Parsonnet, J., Rappuoli, R., 1999. Helicobacterpylori virulence and genetic geography. Science 284, 1328–1333.
[6] Crowe SE, Alvarez L, Dytoc M, et al. Expression ofinterleukin-8 and CD54 by human gastric epithelium afterHelicobacter pylori infection in vitro. Gastroenterology 1995;108: 65-74.
[7] Crabtree JE. Gastric mucosal inflammatory responses toHelicobacter pylori. Aliment PharmacolTher 1996; 10: 29- 23.Crowe SE, Alvarez L, Dytoc M, et al. Expression ofinterleukin-8 and CD54 by human gastric epithelium afterHelicobacter pylori infection in vitro. Gastroenterology 1995;108: 65-74.
[8] Gurbuz I, Akyuz C, Yesilada E, Sener B. Anti-ulcerogenic effect of Momordica charantia L. fruits on various ulcer models in rats. J Ethnopharmacol 2000; 71: 77-82.
[9] Hentschel, E., Brandstatter, G., Dragsics, B., Hirschil, A.M., Nemec, H., Schutze, K.,Taufer, M., Wurzer, H., 1993. Effect of ranitidine and amoxicillin plus metronidazoleon the eradication of Helicobacter pylori and the recurrence of duodenalulcer. The New England Journal of Medicine 328, 308–312.
[10] Hentschel, E., Brandstatter, G., Dragsics, B., Hirschil, A.M., Nemec, H., Schutze, K.,Taufer, M., Wurzer, H., 1993. Effect of ranitidine and amoxicillin plus metronidazoleon the eradication of Helicobacter pylori and the recurrence of duodenalulcer. The New England Journal of Medicine 328, 308–312
[11] Jose, J.K., Y. Kuttan and R. Kuttan, 2001. Antitumor activity of Emblica officinalis . Journal of Ethnopharmacology, 75: 65-69.
[12] Lin, Y.T., Y.I. Kwon, R.G. Labbee and K. Shett, 2005. virus infection on plant pigments. Experimental Inhibition of Heliobacter pylori and associated Biology, 29: 1165-1167. urease by oregano and cranberry phytochemical synergies. Applied and Environmental Microbiology, 71: 8558-8564.
[13] Ndip, R.N., Tarkang, A.E.M., Mbullah, S.M., Luma, H.N., Malongue, A., Ndip, L.M.,Nyongbela, K., Wirmum, C., Efange, S.M.N., 2007. In vitro anti-Helicobacter pyloriactivity of extracts of selected medicinal plants from North West Cameroon.Journal of.Ethnopharmacology 114, 452–457
[14] P.Karthik,et al., 1P.Amudha, 2J.SrikanthStudy on phytochemical profile and anti-ulcerogenic effect of Cayratia pedataLamin albino wistar ratsPharmacologyonline 2: 1017-1029 (2010) Karthik et al.
[15] Park S, Lee DK, Yang CH. Inhibition of fos-jun-DNA complex formation by dihydroguaiaretic acid and in vitro cytotoxic effects on cancer cells. Cancer Letters 1998; 27:
[16] Patil, S. G. Honrao, B. K. Journal of Economic and Taxonomic Botany, 2000, 24(3): 688-694
[17] Pounder RE, Ng D.The prevalence of Helicobacter pylori indifferent countries.Aliment PharmacolTher 1995; 9 (Suppl2): 33-9.
[18] Phillipson, J.D. and M.J.O. Neill, 1987. New leads to the treatment of protozoal infections based on natural product molecules. Acta pharmaceutica Nordica, 1:131-144.
[19] Sepulveda, A.R., Coelho, L.G.V., 2002. Helicobacter pylori and gastric malignancies.Helicobacter 7, 37–42.
[20] Shin, I.S., Masuda, H., Naohide, K., 2004. Bactericidal activity of wasabi (Wasbiajaponica) against Helicobacter pylori. Intentional Journal of Food Microbiology94, 255–261.
[21] Sullivan, P.B., Thomas, J.E., Wight, D.G., et al., 1990.H. pylori infection in Gambian childrenwith chronic diarrhea and malnutrition. Archives of Disease in Childhood65, 189–191.Thompson, C.J., Ernst, E., 2002. Systemic review: herbal medicinal productsfor non-ulcer dyspepsia. Alimentary Pharmacology and Therapeutics 16,1689–1699
[22] Scartezzini, P., F. Antognoni, M.A. Raggi, F. Poli and C. Sabbioni, 2006. Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of Emblica officinalis Gaertn. Journal of Ethnopharmacology, 104: 113-118.
[23] Sairam, K., C.V. Rao, B.M. Dora, K.K. Vijay, V.K. Agarwal and R.K. Goel, 2002. Antiulcerogenic effect of methanolic extract of Emblica officinalis : An experimental study. Journal of Ethnopharmacology, 82: 1-9.
[24] VeeradassRajendran, Indumathy S and Gopal V, Anti-nociceptive activity of Cayratiapedatainexperimental animal models, Journal of Pharmacy Research, 2011,4(3):852-853.
[25] VeeradassRajendran, Rathinambal V and Gopal V, A preliminary study on antiinflammatoryactivity of Cayratiapedataleaves on Wister albino rats, Scholars research library. Der PharmaciaLettre, 2011,3 (2) : 433-437.
[26] Voravathikuchai, S.P. and H. Mitchell, 2008. Inhibitory and killing Activities of MedicinalPlants against Multiple Antibiotic-resistant Helicobacter pylori. Journal of Health Science,54: 81-88.
[27] Wang, Y.C., Huang, T.L., 2005. Screening of anti-Helicobacter pylori herbs derivingfrom Taiwanese folk medicinal plants. FEMS Immunology and Medical Microbiology43, 295–300.
[28] WarrenJR,Marshall BJ. Unidentified curve bacilli on gastric epithelium in active gastricLancet 1983; 1; 1273-1275.
[29] Yesilada, E., Gurbuz, I., Shibata, H., 1999. Screening of Turkish anti-ulcerogenic folk remedies for anti- Helicobacter pylori activity.Journal of Ethnopharmacology 66,289–293.