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Isolation and characterisation of lutein from commelina benghalensis L. Leaves and its sun protection factor activity
Corresponding Author(s) : A. Krishnaveni
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 6 No. 2 (2018): 2018 Volume 6- Issue -2
Abstract
Back ground
Objective
To isolate the lutein from Commelinabenghalensis L. and its sun protection factor determination
Methods
Lutein was isolated by diethyl ether and methanol followed by saponification process. Isolated compound was identified by using UV, NMR and GC-MS spectral analysis. Sun Protection Factor determined by using spectrophotometer. Readings were taken in wavelength ranging from 290 to 320 at 5nm interval and readings were noted down. SPF for isolated lutein can be calculated by applying the formula known as Mansur equation.
Results
The isolated compound shows absorbance maxima at 470, 444 nm it indicate the presence of lutein. UV, NMR, GC-MS studies confirmed the isolated compound is lutein.The SPF number of lutein 50?g/ml is 1.15 ± 0.43. The SPF number of lutein 100?g/ml is 2.38 ± 0.90. The SPF number of lutein 200?g/ml is 4.75 ± 1.79. SPF value for sunscreen above 2 is considered as having good sunscreen activity. Marketed sunscreen lotion having concentration 200 ?g/ml shows SPF value about 0.66±0.006 whereas lutein showed hogh sun protection factor.
Conclusion
Isolated compound lutein possessing good sun screen activity.
Recommendation
Herbal formulations can be prepared and evaluated further with other sensitivity tests
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