Isolation and Characterisation of Cryptolepine from Sidaacutaburm and Its Antimycobacterial Activity

Abstract

Background

Sida acutais a shrub indigenous to pantropical areas, widely distributed in regions and found in pastures, waste lands, cultivated lands, roadsides, lawns and planted forests. Sidaacuta is ethanomedically used as treatment of diuretic, asthma, fever, headache, cough, cold, ulcer, anthelmintic, snake bite, urinary disease, female disorders, sedative, eczema, kidney stone, elephantiasis, testicular swelling, poultice for dandruff, rheumatic affections, facial paralysis, pulmonary tuberculosis and gonorrheae.

Methods

Hydro-alcoholic extract of Sidaacuta was chromatographed (Column) to isolate cryptolepine. The isolatetd SA-I was subjected to various spectral studies such as ultraviolet spectroscopy, infrared spectroscopy, nuclear magnetic resonance spectroscopy and mass spectroscopy. The hydro-alcoholic extract of Sidaacuta and isolated SA-I were evaluated for its anti-mycobacterial studies by Luciferase reporter phage assay method using mycobacterial tuberculosis standard strain of H37RV.

Results

The isolated compound (SA-I) showed the spectral data of cryptolepine. The percentage inhibition of hydro alcoholic extract of Sidaacuta (500 & 1000?g/ml) against mycobacterium tuberculosis showed antimycobacterial effect was found to be 99% in comparison with isoniazid (1ug/ml) 92% and rifampicin (1?g/ml) 96%. Whereas isolated cryptolepine did not any sensitiveness towards mycobacterium tuberculosis.This research indicatecryptolepine did not show any antimycobacterial effect. It specifyantimycobacterial effect of hydro-alcoholic extract of Sidaacuta may be due to the combined nature of cryptolepine with other phytoconstituents.

Conclusion

The present research draws the conclusion that this Sida acuta plant showed extreme antimycobacterial effect, which may be due to the phytoconstituents.