Effect of calcium hydroxide and mineral trioxide aggregate on human PDL fibroblast: an in vitro study
Corresponding Author(s) : Dr Pallavi Giridhar Sonawane
pallu8924@gmail.com
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 6 No. 1 (2018): 2018 Volume 6- Issue -1
Abstract
Background and Aim
The aim of this in vitro study was to investigate the effect of Calcium hydroxide and MTA (Mineral Trioxide Aggregate) on human periodontal ligament fibroblast (PDL).
Method
The cements were divided into three groups Group 1: Calcium hydroxide, Group 2: MTA and Group 3: Calcium hydroxide + MTA. PDL fibroblasts were cultured in above groups. The proliferations of cells were recorded under microscope.
Results
Group 2 showed no lysis of PDL fibroblasts, whereas Group 1 and Group 3 were found slight toxic in nature as they inhibited the PDL cell proliferation.
Conclusion
MTA provided a more favourable environment for PDL fibroblasts adhesion and proliferation
Keywords
Calcium Hydroxide Cement
MTA
Periodontal Ligament Fibroblasts
Periodontal Regeneration
1.
Dr Pallavi Giridhar Sonawane. Effect of calcium hydroxide and mineral trioxide aggregate on human PDL fibroblast: an in vitro study. Int. J. of Allied Med. Sci. and Clin. Res. [Internet]. 2020 Aug. 9 [cited 2026 Apr. 3];6(1):27-31. Available from: https://ijamscr.com/ijamscr/article/view/479
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References
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Textbook of Grossman’s Endodontic Practice 14, 337-338.
[2]. Stratul SI, Schwarz F, Becker J, Willershausen B, Sculean A. Healing of intrabony defects following treatment with an oily calcium hydroxide suspension (Osteoinductal). A controlled clinical study. Clin Oral Investig 10, 2006, 55-60.
[3]. Sigrun Eick et al In vitro-efficacy of oily calcium hydroxide suspension on human alveolar osteoblasts, periodontal ligament fibroblasts and microorganisms. BMC Oral Health 2014.
[4]. Abedi HR et al. The use of mineral trioxide aggregate cement (MTA) as a direct pulp-capping agent, J Endod 22, 1996, 199.
[5]. Dietz G, Bartholmes P, Dietz G-H jr., Roecher W. Calcium Hydroxide and Bone Regeneration. Odontological Aspects of Induced Osteogenesis in Experiment and Clinical Practice. Monography. Byblos Verlag, Munich 1998.
[6]. Al Hiyasat et al, The effect of recasting on the cytotoxicity of base metal alloys. J Prosthet Dent 2005.
Pallavi G S et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-6(1) 2018 [27-31]
31
[7]. Macedo de Menezes et al, Cytotoxicity of polycarbonate orthodontic brackets. Braz J Oral Sci April/june 8, 2009, 270-6.
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[10]. Cytotoxic Effects of Various Mineral Trioxide Aggregate Formulations, Calcium-Enriched Mixture and a New Cement on Human Pulp Stem Cells. Iran Endod J 9, 2014, 271–276.
[11]. De Deus G, Ximenes R, Gurgel-Filho ED, Plotkowski MC, Coutinho-Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J 38(9), 2005, 604–9.
[12]. P. Yan et al. Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts. International Endodontic Journal 43, 2010, 1116–1121. [13]. Camp et al, Adhesion of Human Fibroblasts to Root-End–Filling Materials. J Endod 29, 2003, 602-7.
[14]. Rhythm Bains, Management of pulpal floor perforation and grade II Furcation involvement using mineral trioxide aggregate and platelet rich fibrin: A clinical report.Contemp Clin Dent 3, 2012, 223-227.
[15]. Dietz G, Bartholmes P, Dietz G-H jr., Roecher W. Calcium Hydroxide and Bone Regeneration. Odontological Aspects of Induced Osteogenesis in Experiment and Clinical Practice. Monography. Byblos Verlag, Munich. 7, 1998, 156-164.
[16]. Kasaj A, Willershausen B, Jewszyk N, Schmidt M: Effect of an oily calcium hydroxide suspension (Osteoinductal) on human periodontal fibroblasts. An in vitro study. Eur J Med Res 12(6), 2007, 268-272
References
Textbook of Grossman’s Endodontic Practice 14, 337-338.
[2]. Stratul SI, Schwarz F, Becker J, Willershausen B, Sculean A. Healing of intrabony defects following treatment with an oily calcium hydroxide suspension (Osteoinductal). A controlled clinical study. Clin Oral Investig 10, 2006, 55-60.
[3]. Sigrun Eick et al In vitro-efficacy of oily calcium hydroxide suspension on human alveolar osteoblasts, periodontal ligament fibroblasts and microorganisms. BMC Oral Health 2014.
[4]. Abedi HR et al. The use of mineral trioxide aggregate cement (MTA) as a direct pulp-capping agent, J Endod 22, 1996, 199.
[5]. Dietz G, Bartholmes P, Dietz G-H jr., Roecher W. Calcium Hydroxide and Bone Regeneration. Odontological Aspects of Induced Osteogenesis in Experiment and Clinical Practice. Monography. Byblos Verlag, Munich 1998.
[6]. Al Hiyasat et al, The effect of recasting on the cytotoxicity of base metal alloys. J Prosthet Dent 2005.
Pallavi G S et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-6(1) 2018 [27-31]
31
[7]. Macedo de Menezes et al, Cytotoxicity of polycarbonate orthodontic brackets. Braz J Oral Sci April/june 8, 2009, 270-6.
[8]. Textbook of oral cells and tissues.
[9]. S. N. Al-Haj Ali,S. H. Al-Jundi, D. J. Ditto. In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts. Eur Arch Paediatr Dent 2014.
[10]. Cytotoxic Effects of Various Mineral Trioxide Aggregate Formulations, Calcium-Enriched Mixture and a New Cement on Human Pulp Stem Cells. Iran Endod J 9, 2014, 271–276.
[11]. De Deus G, Ximenes R, Gurgel-Filho ED, Plotkowski MC, Coutinho-Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J 38(9), 2005, 604–9.
[12]. P. Yan et al. Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts. International Endodontic Journal 43, 2010, 1116–1121. [13]. Camp et al, Adhesion of Human Fibroblasts to Root-End–Filling Materials. J Endod 29, 2003, 602-7.
[14]. Rhythm Bains, Management of pulpal floor perforation and grade II Furcation involvement using mineral trioxide aggregate and platelet rich fibrin: A clinical report.Contemp Clin Dent 3, 2012, 223-227.
[15]. Dietz G, Bartholmes P, Dietz G-H jr., Roecher W. Calcium Hydroxide and Bone Regeneration. Odontological Aspects of Induced Osteogenesis in Experiment and Clinical Practice. Monography. Byblos Verlag, Munich. 7, 1998, 156-164.
[16]. Kasaj A, Willershausen B, Jewszyk N, Schmidt M: Effect of an oily calcium hydroxide suspension (Osteoinductal) on human periodontal fibroblasts. An in vitro study. Eur J Med Res 12(6), 2007, 268-272
[2]. Stratul SI, Schwarz F, Becker J, Willershausen B, Sculean A. Healing of intrabony defects following treatment with an oily calcium hydroxide suspension (Osteoinductal). A controlled clinical study. Clin Oral Investig 10, 2006, 55-60.
[3]. Sigrun Eick et al In vitro-efficacy of oily calcium hydroxide suspension on human alveolar osteoblasts, periodontal ligament fibroblasts and microorganisms. BMC Oral Health 2014.
[4]. Abedi HR et al. The use of mineral trioxide aggregate cement (MTA) as a direct pulp-capping agent, J Endod 22, 1996, 199.
[5]. Dietz G, Bartholmes P, Dietz G-H jr., Roecher W. Calcium Hydroxide and Bone Regeneration. Odontological Aspects of Induced Osteogenesis in Experiment and Clinical Practice. Monography. Byblos Verlag, Munich 1998.
[6]. Al Hiyasat et al, The effect of recasting on the cytotoxicity of base metal alloys. J Prosthet Dent 2005.
Pallavi G S et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-6(1) 2018 [27-31]
31
[7]. Macedo de Menezes et al, Cytotoxicity of polycarbonate orthodontic brackets. Braz J Oral Sci April/june 8, 2009, 270-6.
[8]. Textbook of oral cells and tissues.
[9]. S. N. Al-Haj Ali,S. H. Al-Jundi, D. J. Ditto. In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts. Eur Arch Paediatr Dent 2014.
[10]. Cytotoxic Effects of Various Mineral Trioxide Aggregate Formulations, Calcium-Enriched Mixture and a New Cement on Human Pulp Stem Cells. Iran Endod J 9, 2014, 271–276.
[11]. De Deus G, Ximenes R, Gurgel-Filho ED, Plotkowski MC, Coutinho-Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J 38(9), 2005, 604–9.
[12]. P. Yan et al. Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts. International Endodontic Journal 43, 2010, 1116–1121. [13]. Camp et al, Adhesion of Human Fibroblasts to Root-End–Filling Materials. J Endod 29, 2003, 602-7.
[14]. Rhythm Bains, Management of pulpal floor perforation and grade II Furcation involvement using mineral trioxide aggregate and platelet rich fibrin: A clinical report.Contemp Clin Dent 3, 2012, 223-227.
[15]. Dietz G, Bartholmes P, Dietz G-H jr., Roecher W. Calcium Hydroxide and Bone Regeneration. Odontological Aspects of Induced Osteogenesis in Experiment and Clinical Practice. Monography. Byblos Verlag, Munich. 7, 1998, 156-164.
[16]. Kasaj A, Willershausen B, Jewszyk N, Schmidt M: Effect of an oily calcium hydroxide suspension (Osteoinductal) on human periodontal fibroblasts. An in vitro study. Eur J Med Res 12(6), 2007, 268-272