Comparative Evaluation of GTR Membranes to Assess Their Bacterial Activity in Vitro
Corresponding Author(s) : Dr Pallavi Giridhar Sonawane
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 6 No. 1 (2018): 2018 Volume 6- Issue -1
Abstract
Background Periodontal regeneration is a reproduction or construction of a lost or injured part in such a way that the architecture and function of the lost or injured tissues are completely restored. Guided tissue regeneration has been used to regain lost periodontal attachment around teeth. Microorganism can adhere to and colonize exposed membranes leading to the development of nidus of infection. Aim of this study is to evaluate adherence of bacteria Porphyromonas gingivalis over Amnion, Chorion and Collagen GTR membrane.
Methodology The membranes were placed in tubes containing broth cultures and identical concentrations (1×10 8) of these bacteria at 37?C. At 4, 6,12 and 24 hrs. The bacterial cultures were decanted and the membranes in the tubes were agitated gently, in reduced transfer fluid for 4 times to remove non adherent bacteria. Each tube was sonicated for 30 sec in 10 ml RTF to detach adherent bacteria. The detached adherent bacteria were counted using a Petroff-Hausser chamber.
Result P. gingivalis can adhere to Chorion, Amnion and Collagen membranes at interval of 2hrs, 4 hrs and 24 hrs.
Conclusion
P.gingivalis has higher affinity of adherence towards Chorion as compared to Amnion and Collagen membranes.
Keywords
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Melcher A.H. On the repair potential of periodontal tissues. J Periodontol 47, 1976, 256-60.
[2]. Karring T, Cortellini P. Regenerative therapy: furcation defects. Periodontology 2000 19, 1999, 115-137. [3]. Sture Nyman, Jan Gottlow, Thorkild Karring, Jan Lindhe.The regenerative potential of the periodontal ligament.An experimental study in the monkey.J Clin Periodontology 9(3), 1982, 257–265.
[4]. Karring T, Nyman S, Gottlow J, Laurell L. Development of the biological concept of guided tissue regeneration – animal and human studies. Periodontology 2000 1, 1993, 26-35.
[5]. Newman MG. The role of infection and anti-infection treatment in regenerative therapy. J Periodontol 64, 1993, 1166-1170.
[6]. Brecx M, Theilade J, Attstrom R. An ultrastructural quantitative study of the significance of microbial multiplication during early plaque growth. J Periodont Res 18, 1983, 177-186.
[7]. Selvig KA, Nilveus RE, Fitzmorris L, Kersten B, Khorsandi SS. Scanning electron microscopic observations of cell populations and bacterial contamination of membranes used for guided periodontal tissue regeneration in humans. J Periodontol 61, 1990, 515-520.
[8]. Bragd L, Dahlen G, Wikström M, Slots J. The capability of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius to indicate progressive Periodontitis: A retrospectivestudy. J ClinPeriodontol 14, 1987, 95-99.
[9]. Selvig KA Machtet EE, Cho MI, Dunford R, Norderyd J, Zambón JJ, Genco RJ. Clinical, microbiological and histological factors which influence the success of regenerative periodontal therapy. J Periodontol 65, 1994, 154-161.
[10]. Gottlow I, Nyman S, KarringT, LindheI. New attachment formation as the result of controlled tissue regeneration. J ClinPeriodontol 1, 1984, 494-503.
[11]. Anton Sculean, Soren Jepsen. Biometarials for the reconstructive therapy for infrabony defects.J Periodontol 6, 1986, 45-48.
[12]. Tinti C, Vincenzi G, Guided tissue regeneration in the treatment of human facial recession. J Clin Periodontol 63, 1990, 554-560.
[13]. Mayrand D, Grenier D. Detection of collagenase activity in oral bacteria. J Microbiol 31, 1985, 134-138.
Pallavi G S et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-6(1) 2018 [22-26]
26
[14]. Passariello C et al. Periodontal regeneration procedures may induce colonization by glycocalyx-producing bacteria. Med Micro Immunol 180, 1991, 67-72.
[15]. Selvig KA et al. Regeneration surgery of intrabony periodontal defects using ePTFE barrier membranes: Scanning electron microscopic evaluation of retrieved membranes versus clinical healing. J Periodontol 63, 1992, 974-978.
References
[2]. Karring T, Cortellini P. Regenerative therapy: furcation defects. Periodontology 2000 19, 1999, 115-137. [3]. Sture Nyman, Jan Gottlow, Thorkild Karring, Jan Lindhe.The regenerative potential of the periodontal ligament.An experimental study in the monkey.J Clin Periodontology 9(3), 1982, 257–265.
[4]. Karring T, Nyman S, Gottlow J, Laurell L. Development of the biological concept of guided tissue regeneration – animal and human studies. Periodontology 2000 1, 1993, 26-35.
[5]. Newman MG. The role of infection and anti-infection treatment in regenerative therapy. J Periodontol 64, 1993, 1166-1170.
[6]. Brecx M, Theilade J, Attstrom R. An ultrastructural quantitative study of the significance of microbial multiplication during early plaque growth. J Periodont Res 18, 1983, 177-186.
[7]. Selvig KA, Nilveus RE, Fitzmorris L, Kersten B, Khorsandi SS. Scanning electron microscopic observations of cell populations and bacterial contamination of membranes used for guided periodontal tissue regeneration in humans. J Periodontol 61, 1990, 515-520.
[8]. Bragd L, Dahlen G, Wikström M, Slots J. The capability of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius to indicate progressive Periodontitis: A retrospectivestudy. J ClinPeriodontol 14, 1987, 95-99.
[9]. Selvig KA Machtet EE, Cho MI, Dunford R, Norderyd J, Zambón JJ, Genco RJ. Clinical, microbiological and histological factors which influence the success of regenerative periodontal therapy. J Periodontol 65, 1994, 154-161.
[10]. Gottlow I, Nyman S, KarringT, LindheI. New attachment formation as the result of controlled tissue regeneration. J ClinPeriodontol 1, 1984, 494-503.
[11]. Anton Sculean, Soren Jepsen. Biometarials for the reconstructive therapy for infrabony defects.J Periodontol 6, 1986, 45-48.
[12]. Tinti C, Vincenzi G, Guided tissue regeneration in the treatment of human facial recession. J Clin Periodontol 63, 1990, 554-560.
[13]. Mayrand D, Grenier D. Detection of collagenase activity in oral bacteria. J Microbiol 31, 1985, 134-138.
Pallavi G S et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-6(1) 2018 [22-26]
26
[14]. Passariello C et al. Periodontal regeneration procedures may induce colonization by glycocalyx-producing bacteria. Med Micro Immunol 180, 1991, 67-72.
[15]. Selvig KA et al. Regeneration surgery of intrabony periodontal defects using ePTFE barrier membranes: Scanning electron microscopic evaluation of retrieved membranes versus clinical healing. J Periodontol 63, 1992, 974-978.