Antioxidant and Wound Healing Property of Polyherbal Ointment of Nepalese Medicinal Plants

Abstract

Methanolic extracts of Aloe vera, Bauhinia variegate, Centellaasiatica, Cuscuta reflex, Chromalaenaadarata, Cynodondactlon,Myricaesculenta, Nyctanthes arbor tritis, Psidiumguajava, Rhododendron arboretum, Ficuslacorpysus, Pyruspashia, Bombaxceiba were evaluated for their total tannins and total phenolics. Among them, Bauhinia variegata, Myricaesculenta, Rhododendron arboreum, PyruspashiaandPsidiumguajavawere found to have highest tannin content. These plants were further subjected to evaluate the antioxidant activity by DPPH assay. Bauhinia variegata, Rhododendron arboreum, andMyricaesculentawere formulated into 10% w/w ointment in the ratio of 1:1:2 respectively. In excision wound model, 9 days observation showed that wound was totally healed in herbal ointment treated rats while 2.72%, 4.5%, and 5.73% wound area was found remaining in Framycetin treated, blank and control group of rats, respectively.These traditional medicinal plants showed the significant wound healing activity in animal models, which justifies their use in traditional practice in Nepal.