In Vitro Antioxident and Free Radical Scavenging Studies of Carissa Spinarum L

Abstract

Free radicals are implicated for many diseases including cancer, diabetes mellitus, arthritis, ageing etc. In the treatment of these diseases, antioxidant therapy has gained very importance. Various extract of Carissa spinarum L was studied for its in vitro antioxidant activity used different models viz. ABTS radical scavenging, DPPH radical scavenging, H2O2, Hydroxyl radical, lipid peroxidation and Super oxide. The results were analyzed statistically by the regression methods. The scavenging effect of plant extracts and standard (L.ascorbic acid) on the various scavenging methods in the following order L-ascorbic acid >Ethanol>Ethyl acetate>Chloroform>Pet ether. Its antioxidant activity was estimated by IC50 value and the values are 102.6 to 244.2 ?g/ml (ABTSradical scavenging), 200.41 to354.42 ?g/ml (DPPH radical scavenging), 152.31to 236.12 ?g/ml (H2O2), 188.74 to 296.41 ?g/ml (Hydroxyl radical), 142.12 to291.41 ?g/ml (Lipid peroxidation), and 157.42 to 306.16 ?g/ml (Super oxide). The results clearly indicate that Carissa spinarum L has a significant potential to use as a natural antioxidant agent