Prevalence of gram positive and gram negative organisms in various clinical samples

Abstract

Background

The present work was aimed to depict the prevalence of Gram positive and Gram negative organisms isolated from different clinical samples.

Materials and Methods

Different pathogenic organisms were isolated from Males and females of different clinical samples such as Midstream Urine, Miscellaneous (Sputum, Pus, HVS, Body fluids, endotracheal aspirates, Eye swabs) and Blood samples, during the period of October 2015 to December 2015. Samples were processed and identification of isolates were done by gram staining, biochemical test. Antibiotic sensitivity testing of isolated pathogenic organisms was done by a disk diffusion technique using Mueller Hinton Agar plates according to CLSI guidelines.

Results

Out of total 770 samples analyzed, 414 samples showed the presence of bacteria, whereas 356 samples showed no growth of organisms on culture medium. Microbial growth revealed the presence of 11 different organisms which are reported here (Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis, Citrobacter koseri, Proteus mirabilis, Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus pyogenes).

Conclusion

The present study provided the most needed information on the prevalence of both Gram positive and Gram negative bacteria which were also responsible for infections in Urine, Miscellaneous and Blood samples.