Method Validation For Estimation Of Atenolol and Indapamide in Tablet Formulation

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Vol. 13 No. 3 (2025): 2025 Volume -13 - Issue 3

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August 4, 2025
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Method Validation For Estimation Of Atenolol and Indapamide in Tablet Formulation

Neha Nahid
Research Scholar at Mewar University, Gangrar Chittorgarh (Raj.) 312901
Tanya Sharma
Assistant Professor at Mewar University, Gangrar Chittorgarh (Raj.) 312901
Aryan G. Verma
Research Scholar at Mewar University, Gangrar Chittorgarh (Raj.) 312901
Kulbir Singh
Research Scholar at Mewar University, Gangrar Chittorgarh (Raj.) 312901
Basharat Nawaz
Assistant Professor at Mewar University, Gangrar Chittorgarh (Raj.) 312901

Corresponding Author(s) : Tanya Sharma

kulbirnaag10@gmail.com

International Journal of Allied Medical Sciences and Clinical Research, Vol. 13 No. 3 (2025): 2025 Volume -13 - Issue 3

Abstract

This study focuses on the method validation for the estimation of Atenolol and Indapamide in tablet formulation using High-Performance Liquid Chromatography (HPLC). The method involves using a mobile phase of methanol:water (55:45) with 0.1% v/v Ammonium Hydroxide, a Phenomenex C18 analytical column, and UV detection at 260nm. The method was validated for parameters such as selectivity, specificity, linearity, precision, limit of detection, and limit of quantification. The results showed that the method is reproducible, accurate, and precise for the estimation of Atenolol and Indapamide in tablet formulation.

Keywords

Atenolo Indapamide High Performance Liquid Chromatography (HPLC) UV Detection Methanol Ammonium Hydroxide Pharmacokinetics Pharmacodynamics Bioavailability Standardization Adverse Effects Contraindications
1.
Neha Nahid, Tanya Sharma, Aryan G. Verma, Kulbir Singh, Basharat Nawaz. Method Validation For Estimation Of Atenolol and Indapamide in Tablet Formulation. Int. J. of Allied Med. Sci. and Clin. Res. [Internet]. 2025 Aug. 4 [cited 2025 Sep. 20];13(3):482-9. Available from: https://ijamscr.com/ijamscr/article/view/1654
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