Development of a New Chromatographic Method for Estimation of Paracetamol and Metoclopramide Hcl in Bulk and Pharmaceutical Dosage Form by HPLC
Corresponding Author(s) : Kucharlapati Madhu Hasitha Devi
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 11 No. 3 (2023): 2023 Volume -11 - Issue 3
Abstract
A new simple, accurate, economic, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Paracetamol and Metoclopramide HCl, in its pure form as well as in pharmaceutical dosage form. Chromatography was carried out on X bridge C18 (4.6×150mm) 5 µ column using a mixture of Methanol: Phosphate Buffer pH-3.6 (30:70v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 260nm. The retention time of the Paracetamol and Metoclopramide HCl was 2.669, 3.855±0.02min respectively. The method produce linear responses in the concentration range of 10-50µg/ml of Paracetamol and 10-50µg/ml of Metoclopramide HCl. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
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References
Dr. Kealey, Haines PJ. Analytical chemistry. 1st ed. Bios Publisher; 2002. P. 1-7.
BraithWait A, Smith FJ. Chromatographic methods. 5th ed. Kluwer Academic Publishers; 1996. P. 1-2.
Weston A, Phyllisr. Brown, HPLC principle and practice. 1st ed. Academic press; 1997. P. 24-37.
Kazakevich Y, Lobrutto R. HPLC for pharmaceutical scientists. 1st ed. Wiley Interscience A JohnWiley & Sons, Inc Publishing House; 2007. P. 15-23.
Chromatography [online]. Wikipedia. Available from: http://en.wikipedia.org/wiki/Chromatography
Meyer VR. Practical high-performance liquid chromatography. 4th ed. England: John Wiley & Sons Ltd; 2004. P. 7-8.
Sahajwalla CG a new drug development. Vol. 141. New York: Marcel Dekker, Inc; 2004. P. 421-6.
Introduction to column [online]. Available from: http://amitpatel745.topcities.com/index_files/study/columncare.pdf.
Detectors used in HPLC (online). Available from: http://wiki.answers.com/Q/What_detectors_are_used_in_HPLC.
Detectors [online]. Available from: http://hplc.chem.shu.edu/NEW/HPLC_Book/Detectors/det_uvda.html.
Draft ICH. Guidelines on Validation of Analytical Procedures Definitions and terminology. Fed Regist. 1995;60:1126.
Code. Q2B, validation of analytical procedures; methodology. ICH harmonized tripartite guidelines. 1996:1-8.
Introduction to analytical method validation [online], available from. Available from: http://www.standardbase.hu/tech/HPLC%20validation%20PE.pdf.
Data elements required for assay validation [online] available from. Available from: http://www.labcompliance.com/tutorial/methods/default.aspx.
Snyder LR practical HPLC method development. 2nd ed. New York: John Wiley & sons; 1997. P. 180-2.
Skoog DA, West DM, Holler FJ. Introduction of analytical chemistry. Sounder college of publishing, Harcourt Brace college publishers; 1994. P. 1-5.