Evaluation of Anticancer Activity of Various Extracts of Fruit of Garcinia Pedunculata Using Cervical Cancer Cell Line by Invitro Methods
Corresponding Author(s) : G. Suresh Kumar
suresh.niper12@gmail.com
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 11 No. 1 (2023): 2023 Volume -11 - Issue 1
Abstract
The present study was undertaken to investigate invitro anticancer activity of various extracts of Garcinia pedunculata using HeLA cell line. Phytochemical analysis of acetone, ethanol and aqueous extracts of Garcinia pedunculata revealed the presence of proteins, terpenoids, tannins, steroids, phenols and flavonoids. In this study, ethanolic extract of Garcinia pedunculata showed cytotoxic activity in cervical cancer cell line HeLa by MTT assay. The Apoptotic effect was confirmed by loss of membrane integrity, chromatin condensation, leakage of cytoplasmic contents and fragmentation of DNA by microscopic methods. Its apoptotic and anti cancer effect may be due to up regulation of genes like p53 and down regulation of gene Bcl-2, which was confirmed by RT-PCR. The ethanolic extract of Garcinia pedunculata possess anti-cancer effect and for future perspective, it can be further confirmed by isolating the compounds responsible for the activity and studying the exact mechanism by which the plant possess this activity and confirm the results using in vivo animal models.
Keywords
Anticancer Activity, Garcinia Pedunculata, Cervical Cancer, Invitro Methods
1.
S. K Godasu, Praveen Gujjula, G. Anusha, Nimma.vijayarekha, D. Varun, G. Suresh Kumar. Evaluation of Anticancer Activity of Various Extracts of Fruit of Garcinia Pedunculata Using Cervical Cancer Cell Line by Invitro Methods. Int. J. of Allied Med. Sci. and Clin. Res. [Internet]. 2023 Mar. 30 [cited 2025 Sep. 20];11(1):82-91. Available from: https://ijamscr.com/ijamscr/article/view/1307
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References
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References
1. J. Ferlay, H. R. Shin, F. Bray, D. Forman, C. Mathers, and D. M. Parkin, “Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008,” International Journal of Cancer, vol. 127, no. 12, pp. 2893–2917, 2010.
2. R. Siegel, D. Naishadham, and A. Jemal, “Cancer statistics, 2013,” CA: A Cancer Journal for Clinicians, vol. 63, no. 1, pp. 11–30, 2013.
3. K. S. Tewari, M.W. Sill, H. J. Long et al., “Improved survival with bevacizumab in advanced cervical cancer,” New England Journal of Medicine, vol. 370, no. 8, pp. 734–743, 2014.
4. S.-M. Wang and Y.-L. Qiao, “Implementation of cervical cancer screening and prevention in China—challenges and reality,” Japanese Journal of Clinical Oncology, vol. 45, no. 1, pp. 7–11, 2015.
5. K. Sak, “Chemotherapy and dietary phytochemical agents,” Chemotherapy Research and Practice, vol. 2012, Article ID 282570, 11 pages, 2012.
6. R. Baskar, J. Dai, N. Wenlong, R. Yeo, and K. Yeoh, “Biological response of cancer cells to radiation treatment,” Frontiers in Molecular Biosciences, vol. 1, p. 24, 2014.
2. R. Siegel, D. Naishadham, and A. Jemal, “Cancer statistics, 2013,” CA: A Cancer Journal for Clinicians, vol. 63, no. 1, pp. 11–30, 2013.
3. K. S. Tewari, M.W. Sill, H. J. Long et al., “Improved survival with bevacizumab in advanced cervical cancer,” New England Journal of Medicine, vol. 370, no. 8, pp. 734–743, 2014.
4. S.-M. Wang and Y.-L. Qiao, “Implementation of cervical cancer screening and prevention in China—challenges and reality,” Japanese Journal of Clinical Oncology, vol. 45, no. 1, pp. 7–11, 2015.
5. K. Sak, “Chemotherapy and dietary phytochemical agents,” Chemotherapy Research and Practice, vol. 2012, Article ID 282570, 11 pages, 2012.
6. R. Baskar, J. Dai, N. Wenlong, R. Yeo, and K. Yeoh, “Biological response of cancer cells to radiation treatment,” Frontiers in Molecular Biosciences, vol. 1, p. 24, 2014.