Razvy et al. studied the antibacterial activity is measured by zone of inhibition (mm). Totally four bacterial strains (two gram positive S.aureus, B.subtilis and two gram negative bacteria P. aeurogenosa, E.coli. Ethanolic extract of VetiveriaZizanioides is known to Posses flavonoids, alkaloids, terpenoids, saponins, tannins and phenols which either individually or combination exert antimicrobial activity. The study showed that EEVZ inhibited gram negative bacteria than gram positive bacteria. Flavonoids are found to be effective antimicrobial substance against a wide range of microorganisms, probably due to their ability to complex with extra cellular and soluble proteins and to complex with bacterial cell wall; more lipophilic flavonoids may disrupt microbial membrane. Antibacterial activity of tannins may be related to their ability to inactivate microbial adhesion enzymes and cell envelope transport proteins, they also complex with polysaccharides. The presence of tannins present in the roots of Vetiveriazizanioides implied that tannin may be the active compound which may be responsible for in vitro antibacterial activity in this study. Tannin in the plant extract was found to possess antibacterial [21].

Nuchuchua O et al. reported that the nano emulsions composed of citronella oil, hairy basil oil, and vetiver oil with mean droplet sizes ranging from 150 to 220 nm were prepared and investigated both in vitro and in vivo. Larger emulsion droplets (195-220 nm) shifted toward a smaller size (150-160 nm) after high-pressure homogenization and resulted in higher release rate. It was proposed that thin films obtained from the nanoemulsions with smaller droplet size would have higher integrity, thus increasing the vaporization of essential oils and subsequently prolonging the mosquito repellant activity. In the laboratory oviposition deterrent test, the root extract of Vetiveriazizanioidesat each concnentration greatly reduced the number of eggs deposited by the gravid Anaphelesstephens[22].

Sridhar SR et al. Studied The antifungal activity of ethanol and aqueous extracts of Vetiveriazizanioides. Ethanol and aqueous extracts of VetiveriaZizanioideswere prepared. Standard cultures of Asperigulls Nigar, Asperigulls Clavatus and Candida Albicanus were used for the study. The antifungal tests were conducted by using agar well plate method nystatin and Griseofulvin were used as standard[23]

Chaudhary GD et al. studied the VetiveriaZizanioides L. Nash (Family: Poaceae) root (intact and spent) extracts and fractions were evaluated for Anti mycobacterial activity against Mycobacterium tuberculosis H(37)Rv and H(37)Ra strains using radiometric BACTEC 460 TB system.The ethanolic extract of intact as well as spent root was showed potent Antituberculosis activity at a minimum concentration of 500μg/ml. The hexane fraction also showed antibacterial action by recording continuous decline in growth index (GI) of Mycobacterium tuberculosis at 50μg/ml. It was further more observed that root extract and hexane fraction showed activity even after the extraction of essential oil by hydro distillation[24].

Kim H et al. Reported that the Free radicals induce numerous diseases by lipid peroxidation and DNA damage. It has been reported that some of the extracts from plants possess antioxidant properties capable of scavenging free radicals in vivo. Vetiveriazizanioides is a densely tufted grass which is widely used as a traditional plant for aromatherapy, to relieve stress, anxiety, nervous tension and insomnia. In this regard, the roots of Vetiverazizanioides was extracted with ethanol and used for the evaluation of various in vitro antioxidant activities such as reducing power ability, superoxide anion radical scavenging activity, deoxyribose degradation assay, total antioxidant capacity, total Phenolics and total flavonoid composition [25].

Hassan et al. studied the Methanolic extract of Vetiveriazizanioides Linn shows hepato protective at the dose 300-500mg/kg p o damage induced by ethanol 20% at the dose of 3.76gm/kg p o [26].

The ethanolic extract of Vetiveriazizanioides posses antidepressant activity and the combination of Fluxetine and ethanolic extract of Vetiveriazizanioides is effective against tail suspension test and force swim test induced depressive behaviour [27].

Karan Sk et al. Reported that The effect of root extract of Vetiveriazizanioides in normal fasted rats after multiple doses showed significant antidiabetic activity at 2nd and 4th hour after administration compared to diabetic control, result were comparable with standard glibenclamide. The study indicates the ethanolic extract of Vetiveriazizanioidesroots posses better antihyperglyceamic activity than any other extract, in both normal and allaxon induced diabetic rats [28].

Swiss albino mice were taken for the experiment. The animals were made into a group of 3 each, dose of extracts was given according to the body weight (mg/kg), starting dose of 5-5000 mg /kg was given to the first individual animal, no death was occurred, and higher doses were given to next group of animals. The observation shows the various extracts of root of Vetiveriazizanioides was screened for acutetoxicity study for determining the LD50. The results showed that LD50 was found to be 5000mg/kg. 1/10th of LD50 is called as ED50. Therefore its ED50 was found to be 500mg/kg[29].

Elizabeth et al. Reported that anethanolic extract of Vetiveriazizainoides in Wistar rats and Swiss albino mice showed analgesic and anti-inflammatory activity possible mediated through central and peripheral mechanisms[31].

Rajeswari etal. reported that the Vetiver zizanioides roots extracts in STZ-induced diabetic Wistar rats. Results showed significant improved glycemic control, antioxidant and hypo lipidemic properties, together with protective effect against hepatic and renal injury Associated with diabetes [32].