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Recent Advances of Plant Tissue Culture in Pharmaceuticals And Opportunities
Corresponding Author(s) : Vitta kavya
International Journal of Allied Medical Sciences and Clinical Research,
Vol. 9 No. 2 (2021): 2021 Volume - 9 Issue - 2
Abstract
India features a very rich plant biodiversity, many of which are medicinally useful. The rich resource is disappearing at an alarming rate as results of over- exploitation. Rapid agricultural development, increase, urbanization and therefore the indiscriminate collection of medicinal plants from the wild is resulted in an over-exploitation of natural resources. The traditional means of propagation takes an extended time for multiplication and also clonal non uniform. Plant in vitro regeneration may be a biotechnological tool that gives an incredible potential solution for the propagation of endangered and superior genotypes of medicinal plants which might be released to their natural habitat or cultivated on an outsized scale for the pharmaceutical product of interest. After the last four years of intensive research programmes in our laboratory, we are ready to micropropagate a number of the endangered and valuable medicinal plants species of this region. Plant tissue culture refers to growing and multiplication of cells, tissues and organs of plants on defined solid or liquid media under aseptic and controlled environment. The commercial technology is based on micro propagation, during which rapid proliferation is achieved from in system cuttings, axillary buds, and to a limited extent from somatic embryos, cell clumps in suspension cultures and bioreactors.
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References
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3. Brown DCW, Thorpe TA. Crop improvement through tissue culture. World J Microbiol Biotechnol. 1995; 11(4):409-15. doi: 10.1007/BF00364616, PMID 24414749.
4. George EF. Plant propagation by Tissue Culture. Eversley: Eastern Press; 1993.
5. Garcia-Gonzales R, Quiroz K, Carrasco B, Caligari P. Plant tissue culture: Current status, opportunities and challenges. Cien. Investig Agr. 2010; 37(3):5-30.
6. Sagare AP, Lee YL, Lin TC, Chen CC, Tsay HS. Cytokinin-induced somatic embryogenesis and plant regeneration in Corydalis yanhusuo (Fumariaceae) - a medicinal plant. Plant Sci. 2000; 160(1):139-47. doi: 10.1016/s0168-9452(00)00377-0, PMID 11164586.
7. El-Dougdoug KA, El-Shamy MM. Management of viral diseases in banana using certified and virus tested plant material. Afr J Microbiol Res. 2011; 5(32):5923-32.
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12. Rafiq M, Dahot MU, Mangrio SM, Naqvi HA Qarshi IA. In vitro clonalpropagation and biochemical analysis of field established Stevia rebaudiana Bertoni. Pak J Bot. 2007; 39(7):2467-74.
13. Rout GR. Effect of cytokinins and auxin on micropropagation of Clitoria ternateaL. Biol Lett. 2004; 41(1):21-6.
14. Hussain A, Naz S, Nazir H, Shinwari ZK. Tissue culture of black pepper (Piper nigrum L.) in Pakistan. Pak J Bot. 2011; 43(2):1069-78.
15. Murashige T. Plant propagation through tissue cultures. Annu Rev Plant Physiol. 1974; 25(1):135-66. doi: 10.1146/annurev.pp.25.060174.001031.
16. Cassells AC, Doyle BM. Pathogen and biological contamination management: the road ahead. In: Loyola-Vargas VM, Vázquez-Flota F, editors. Plant cell Culture Protocols. New York: Humana Press; 2005. p. 35-50.
17. Husain MK, Anis M. Rapid in vitro multiplication of Melia azedarach L. (amultipurpose woody tree). Acta Physiol Plant. 2009; 31(4):765-72. doi: 10.1007/s11738-009-0290-7.
18. Tilkat E, Onay A, Y?ld?r?m H, Ayaz E. Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L Sci Hortic. 2009; 121(3):361-5. doi: 10.1016/j.scienta.2009.02.007.
19. Marana JP, Miglioranza É, Faria RTd. Estabelecimento in vitro de Jacaratia spinosa (Aubl.) ADC. Sem Ci Agr; 30(2). doi: 10.5433/1679-0359.2009v30n2p271.
20. Garcia R, Morán R, Somonte D, Zaldúa Z, López A, Mena CJ. Sweet potato (Ipomoea batatas L.) biotechnology: perspectives and progress. In: Altman A, editor, Ziv M, plant tissue culture: current status and opportunities 23Shamay I, editors. Plant biotechnology and in vitro biology in 21st century. The Netherlands; 1999. p. 143-6.
21. Singh KK, Gurung B. In vitro propagation of R. maddeni Hook. F. an endangered Rhododendron species of Sikkim Himalaya. Not Bot Horti Agrobot Cluj-Napoca. 2009; 37(1):79-83.
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