Articles

  1. Method development and validation of dabigatran in pharmaceutical dosage form by RP- HPLC method Download Article

    Ajitha A, P.Sandhya Rani, Chandu
    • Article Type: Research Article
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    • Pages (01-08)
    • No of Download = 11

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    A simple precise, accurate method was developed and validated by reversed phase high performance liquid chromatography method used for the estimation of Dabigatran in bulk and pharmaceutical dosage form. It is reversed phase liquid chromatography. The HPLC method has been carried out by using C18 150x4.6mm 5µm column. This method has been developed by using the mobile phase consisting buffer: Acetonitrile 65:35 and the flow rate of 1ml/min by the detection of UV at 330nm. The retention time of the dabigatran is 0.999 min. The runtime is 15min. the linearity was found to be over a concentration of 25%-150% respectively. The accuracy was found to be 98.84 to 100.24%. With a correlation coefficient of0.999.The proposed method can be used for the estimation of the drug in bulk and pharmaceutical formulation. The results of analysis have been validated satisfactorily using recovery studies.

  2. RP-HPLC method development and validation for estimation of doxorubicin and in bulk and pharmaceutical dosage form Download Article

    Monika Renikunta, Dr.T.Rama Mohan Reddy, Dr.Konde Abbulu
    • Article Type: Research Article
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    • Pages (09-16)
    • No of Download = 11

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    A simple, Precised, Accurate method was developed for the estimation of doxorubicin by RP-HPLC technique. Chromatographic conditions used are stationary phase Discovery C18 150mm x 4.6 mm, 5 ,Mobile phase 0.1% OPA: Acetonitrile in the ratio of 45:55 and flow rate was maintained at 0.9ml/min, detection wave length was 234 nm, column temperature was set to 30oC and diluent was mobile phase Conditions were finalized as optimized method. System suitability parameters were studied by injecting the standard six times and results were well under the acceptance criteria. Linearity study was carried out between 25% to150 % levels, R2 value was found to be as 0.999. Precision was found to be 0.6 for repeatability and 0.7 for intermediate precision. LOD and LOQ are 0.085µg/ml and 0.258µg/ml respectively. By using above method assay of marketed formulation was carried out 99.90% was present. Degradation studies of doxorubicin were done, in all conditions purity threshold was more than purity angle and within the acceptable range.

  3. Spectrophotometric method development and validation for the estimation of ticagrelor in bulk and its dosage forms Download Article

    P. Sireesha, A. Ajitha, T. Rama Mohan Reddy
    • Article Type: Research Article
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    • Pages (17-20)
    • No of Download = 11

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    Simple, sensitive, accurate, precise, stability indicating UV spectrophotometric method have been developed for quantitative determination of ticagrelor in bulk and its dosage form. The UV spectrum was scanned between 200-400 nm and 254 nm was selected as maximum wavelength for absorption. Beer’s law was obeyed in the concentration range of 2-10µg/ml were found the method was successfully applied to the pharmaceutical dosage forms containing the above mentioned drug without any interference by excipients. Results of the analysis were validated as per ICH guidelines. This method can be used for the routine and quality control analysis of ticagrelor in raw material and pharmaceutical formulations.

  4. Analysis of the Effect of Phantom CT Scan Diameter Variations on Radiation Dose with IndoseCT Download Article

    Intan Andriani, Wahyu Setia Budi, Heri Sutanto, and Choirul Anam
    • Article Type: Research Article
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    • Pages (21-27)
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    The dose received by a patient who performs a CT scan is influenced by several factors, namely slice thickness, pitch, position and size of the patient, range of scanning and type of scanning. The amount of radiation dose received from the scan also depends on phantom geometry, form and material. The method of calculating a patient's dose on a CT scan is known as the Computed Tomography Dose Index (CTDI). According to research that has already been carried out, it is explained that CTDI represents only the surface dose of the body. SSDE is then used to calculate the central dose of phantom and phantom edge. The purpose of this study was to determine the dosage size for variations in phantom diameter and SSDE values with the IndoseCT program for variations in phantom diameter. The measurement method in this study was carried out using a piranha detector and IndoseCT software. The results of the study found that there were differences in the values of CTDIc, CTDIw, and CTDIvol, each having a range of 0.93% to 147.96%, 26.65% to 62.48%, 11.51% to 41.46%. SSDE values for phantom size 8 cm, 16 cm, 24 cm, 32 cm and 40 cm respectively are as follows: 29 mGy, 23.09 mGy, 16.86 mGy, 12.35 mGy, 9.27 mGy. The conclusion of this study is that the greater the effective diameter of phantom the smaller the SSDE.

  5. Effectiveness of nutritive balls for weight gain among preschool children at selected school Download Article

    Kavitha.P
    • Article Type: Research Article
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    • Pages (28-31)
    • No of Download = 8

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    A study was done to assess the effectiveness of nutritive balls for weight gain among preschool children at selected School, Perambalur. Quasi experimental design was adopted for the study. 30 samples were selected from School through convenient sampling technique & divided into experimental and control group. The demographic data were collected from the preschool children. Weighing machine were used to assess the level of weight gain in preschool children. Significant difference was found between pretest and posttest levels of weight among preschool children in experimental group at <0.001 level. The comparison between experimental group and control group posttest shows that the intervention is effective. There was no significant association between the posttest value of weight and their selected demographic variable.

  6. Analysis on the use of packaged pineapple juice as an alternative for oral negative contrast medium in magnetic resonance enterography (MRE) Download Article

    Ni Putu Rita Jeniyanthi, Sugiyanto, A. Gunawan Santoso, Made Wijaya, Choirul Anwar
    • Article Type: Research Article
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    • Pages (32-39)
    • No of Download = 9

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    Background Oral negative contrast medium used for Abdominal examination made of chemicals has some negative effects on the body and some are difficult to find in the market and are expensive too. Thus, the solution is to conduct a study to find alternative oral negative media in abdominal MRI examination by using fruit ingredients such as, blueberry juice, pineapple juice and tea that contain lots of manganese (Mn) [1] Objective This study by using natural alternative oral negative contrast medium in MRE that is considered to be ready in Indonesia, the taste is acceptable and not cause a negative effect after its use [2, 3] Methods This study was an experimental study with Pretest-Posttest Control Group Design. Data analysis was performed with One Way Anova Test and Repeated Anova with Tukey’s Post Hoc test. Results There was a difference on the digestive system diameter before and after the administration of packaged pineapple juice as an alternative oral negative contrast medium in MRE examination with p value < 0.05 Conclusion p value was 0.043 and the optimal scanning time in MRE image was 20 minutes after the administration of packaged pineapple juice based on the highest Mean Rank with the value of (20.16). Recommendation Packaged pineapple juice with high manganese content can be an alternative oral negative contrast medium in MRE examination with optimal scanning time at 20 minutes after the administration.

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